两种方法提取川芎挥发油指纹图谱对比

Fingerprints of Volatile Oil from Chuanxiong Rhizoma by Two Methods

摘要:

目的: 建立川芎挥发油的气相(GC)指纹图谱,并且比较蒸馏-萃取耦合法和传统蒸馏法所提川芎挥发油的指纹图谱。方法: 蒸馏-萃取耦合法提取川芎挥发油,气相色谱法进行指纹图谱研究,采用AT-5弹性石英毛细管柱(0.32 mm×30 m,0.25 μm),进样口和检测器温度250 ℃,FID检测器,用"中药指纹图谱计算机辅助相似度评价软件"计算相似度。结果: 建立了蒸馏-萃取耦合法提取川芎挥发油的气相(GC)指纹图谱,方法有较好的精密度,重复性,稳定性(RSD<3%)。蒸馏-萃取耦合法所提川芎挥发油和传统蒸馏法所提川芎挥发油指纹图谱中共有成分>98%,共有成分相似度>0.998。结论: 蒸馏-萃取耦合法所提川芎挥发油中藁本内酯比传统蒸馏法所提挥发油中藁本内酯含量增加83.46%,该法所提川芎挥发油的指纹图谱与传统蒸馏法所提川芎挥发油的指纹图谱一致。

Abstract:

Objective: To establish GC fingerprints of volatile oil from Chuanxiong Rhizoma. To compare the consistency of fingerprints of volatile oil from Chuanxiong Rhizoma by distillation coupling solvent extraction and traditional distillation extraction. Method: The volatile oil was extracted from Chuanxiong Rhizoma by distillation coupling solvent extraction.The chromatography fingerprints was established by AT-5 fused-silica capillary (0.32 mm×30 m,0.25 μm)column,injector temperature of 250 ℃, FID detector with temperature at 250 ℃.The similarity was calculated with "TCM fingerprint aided similarity evaluation software". Result: Established GC fingerprints of volatile oil from Chuanxiong Rhizoma.The reproducibility,accuracy and stability of the method were very good in the tests (all RSD<3%).The common components of volatile oil from Ligusticum chuanxiong by distillation coupling solvent extraction and traditional distillation extraction>98%.The similarity of common components was more than 0.998. Conclusion: The ligustilide from Chuanxiong Rhizoma by distillation coupling solvent extraction is 83.46 percent higher than traditional distillation extraction.The fingerprints of volatile oil from Chuanxiong Rhizoma by distillation coupling solvent extraction and traditional distillation extraction is consistent.