HPLC测定复方黄葵颗粒中8种成分

Simultaneous Determination of Eight Components in Compound Huangkui Granules by HPLC

摘要:

目的: 建立HPLC同时测定复方黄葵颗粒中毛蕊异黄酮葡萄糖苷、金丝桃苷、芦丁、异槲皮苷、杨梅素、槲皮素、马钱苷、莫诺苷的方法。方法: 采用Aglient C₁₈色谱柱(4.6 mm×250 mm,5 μm),以乙腈-0.05%甲酸水溶液为流动相进行梯度洗脱,流速 0.8 mL·min^-1,检测波长分别为240,260,360 nm,柱温 30 ℃。结果: 毛蕊异黄酮葡萄糖苷、金丝桃苷、芦丁、异槲皮苷、杨梅素、槲皮素、莫诺苷、马钱苷线性范围分别为3.5～35.0(r=0.999 6),32.09～320.9(r=0.999 7),2.21～22.12(r=0.999 7),18.68～186.9(r=0.999 8),6.32～63.20(r=0.999 7),3.54～35.44(r=0.999 6),12.39～123.9(r=0.999 6), 12.88～128.8 mg·L^-1(r=0.999 6)。平均回收率分别为98.1% (RSD 1.7%),99.7%(RSD 0.6%),98.7%(RSD 2.5%),100.1%(RSD 1.4%),99.7%(RSD 1.0%),99.9%(RSD 2.7%),99.0%(RSD 1.2%),99.7%(RSD 1.3%)。结论: 所建立方法简便、准确,重复性好,可作为复方黄葵颗粒的质量控制。

Abstract:

Objective: To establish an HPLC method for simultaneously determining the contents of calycosin-7-O-β-D-glucoside, hypericin, rutin, isoquercetin, myricetin, quercetin, loganin, morroniide in compound Huangkui granules. Method: The determination was performed on an Aglient C₁₈(4.6 mm×250 mm,5 μm) chromatographic column,the mobile phase was acetonitrile-0.05% formic acid aqueous solution with gradient elution at a flow rate of 1.0 mL·min^-1;the detection wavelength was 240,260 and 360 nm, column temperature was 30 ℃. Result: The calibration curves of calycosin-7-O-β-D-glucoside, hypericin, rutin, isoquercetin, myricetin, quercetin, loganin, morroniide were linear in the ranges of 3.5-35.0(r=0.999 6),32.09-300.9(r=0.999 7),2.21-22.12(r=0.999 7),18.68-186.9(r=0.999 8),6.32-63.20(r=0.999 7),3.54-35.44(r=0.999 6),12.39-123.9(r=0.999 6), 12.88-128.8 mg·L^-1(r=0.999 6),respectively. The average recoveries were 98.1%(RSD 1.7%),99.7%(RSD 0.6%),98.7%(RSD 2.5%),100.1%(RSD 1.4%),99.7%(RSD 1.0%),99.9%(RSD 2.7%),99.0%(RSD 1.2%),99.7%(RSD 1.3%),respectively. Conclusion: The method established is simple, accurate and reproducible, which can be used for the quality control of Huangkui granules.