赤芍、大黄药对提取物中丹皮酚和大黄酸的组织分布研究

Tissue Distribution of Drug-couple Radix Paeoniae Rubra and Rhei Radix et Rhizoma

摘要:

目的: 建立同时测定大鼠组织中丹皮酚和大黄酸含量的高效液相色谱法。方法: 采用Phenomsil BDS C₁₈(4.6 mm×250 mm,5 μm),以乙腈-0.1%磷酸(37:63)为流动相,流速1.0 mL·min^-1,以橙黄决明素为内标,在274 nm下进行检测。结果: 丹皮酚的线性范围为Y=-0.055+0.03X(r=0.993 2),日内和日间的RSD均<4.1%;大黄酸的线性范围为Y=-0.152+0.014X(r=0.993 5),日内和日间的RSD均<4.9%。组织样品的稳定性符合要求。结论: 所建立的大鼠组织中丹皮酚和大黄酸HPLC测定方法,适用于大鼠体内赤芍、大黄药对的组织分布研究。

Abstract:

Objective: To develop an HPLC method for simultaneous quantification of paeonol and rhein in the tissue of rats. Method: The adopted chromatographic column was Phenomsil BDS C₁₈(4.6 mm×250 mm, 5 μm), the mobile phase was acetonitrile-0.1% phosphoric acid aqueous solution (37: 63), the flow rate was 1.0 mL·min^-1, and detection wavelength was set at 274 nm. Methyl alcohol was used to precipitate the protein of the plasma samples and the aurantio-obtusin was used as an internal standard. Result: The linear range of paeonol was Y=-0.055+0.03X(r=0.993 2). The intra-day RSD and inter-day RSD were less than 4.1%. For rhein, the linear range was Y=-0.152+0.014X(r=0.993 5), the intra-day RSD and inter-day RSD were less than 4.9%.The stability of the plasma sample were well acceptable. Conclusion: The developed method for the quantification of paeonol and rhein in rat plasma is suitable for the distribution studies of drug-couple Radix Paeoniae Rubra and Rhei Radix et Rhizoma in the tissue of rats.