丹皮酚对大鼠缺血性心律失常及其心肌细胞miRNA-1表达的影响

Effect of Paeonol on Rats' Ischemic Arrhythmia and miRNA-1 Expression

摘要:

目的: 探讨丹皮酚对大鼠缺血性心律失常及其心肌细胞miRNA-1表达的影响。方法: SPF级SD大鼠,按体重随机分为正常组、模型组、阳性药组(盐酸普萘洛尔注射液2 mL·kg^-1)、丹皮酚组(6,3,1 mg·kg^-1)。除正常组外,其余各组以5 μg·kg^-1剂量尾静脉注射乌头碱生理盐水溶液制作缺血性心律失常模型。给药后记录30 min内各实验组大鼠心电图变化,早搏(VPB)次数和首次出现时间,以及室速(VT)和室颤(VF)次 数和持续时间。实验结束后剖取心脏,测定心肌梗死面积,并用RT-qPCR法测定心肌细胞miRNA-1的mRNA表达。结果: 与正常组比较,模型组出现缺血性心率失常,VPB,VT和VF频数明显增加(P<0.05,P<0.01),VPB首发时间明显缩短(P<0.05),VT和VF持续时间明显增长(P<0.05),心肌梗死面积明显增加(P<0.01),明显增加心肌细胞miRNA-1的mRNA表达(P<0.01);给药治疗后,与模型组比较,丹皮酚可明显改善大鼠缺血性心律失常,明显减少VPB,VT和VF频数(P<0.05,P<0.01),明显推迟VPB首发时间(P<0.05),明显缩短VT和VF持续时间(P<0.05,P<0.01),明显减少心肌梗死面积(P<0.05,P<0.01),明显降低心肌细胞miRNA-1的mRNA表达(P<0.05,P<0.01)。结论: 丹皮酚对缺血性心律失常具有明显地改善作用,并能明显的降低心肌细胞miRNA-1的mRNA表达。

Abstract:

Objective: To investigate the effect of paeonol on ischemic arrhythmia and the expression of miRNA-1 of rats. Method: SPF SD rats were randomly divided into the normal group, the model group, the positive group (propranolol hydrochloride injection 2 mL·kg^-1) and the paeonol group (6, 3, 1 mg·kg^-1). The ischemic arrhythmia models were induced by injecting 5 μg·kg^-1dose of aconitine intravenously. ECG changes of each group were recorded, premature beat (VPB) frequency and time for the first time, and ventricular tachycardia (VT) and ventricular fibrillation (VF) frequency and duration were recorded. Hearts were taken out on the end of the experiment, myocardial infarct sizes were measured, and the expression of miRNA-1 in myocardial cells was measured by RT-qPCR method. Result: Compared with the normal group, the ischemic arrhythmia was occurred, the frequencies of VPB, VT and VF increased, the VPB time for the first time shortened, the VT and VF durations increased, myocardial infarct sizes and miRNA-1 expression increased in the model group (P<0.05, P<0.01).The above indicators had good improvement in the paeonol group(P<0.05,P<0.01). Conclusion: Paeonol has an obvious effect in treating ischemic arrhythmias.