腰痹通胶囊对大鼠腰椎神经根压迫致腰椎间盘突出模型药效及炎性因子的影响

Efficiency and Influence of Inflammatory Cytokines by Yaobitong Capsule in Rat Lumbar Disc Herniation Model

摘要:

目的: 建立大鼠腰椎间盘突出模型,观察腰痹通胶囊(YBT)对该模型的药效及血清炎性因子的影响。方法: 利用硅胶片模拟腰椎间盘突出,压迫大鼠L₅神经根,建立大鼠腰椎间盘突出动物模型,并随机分为模型组、阳性药组(腰痛宁胶囊0.16 g·kg^-1)和YBT治疗3个剂量(0.17,0.34,0.68 g·kg^-1)组,另设正常组,给药30 d后,检测大鼠左后肢压痛痛阈、血清肿瘤坏死因子-α(TNF-α),白介素-1β(IL-1β),白介素-6(IL-6)及神经根病理变化。结果: 成功复制大鼠腰椎间盘突出模型。与正常对照组比较,模型组大鼠左后肢压痛痛阈明显降低(P<0.05),血清TNF-α,IL-1β,IL-6含量明显升高(P<0.01)、神经根有明显病变;YBT 0.34,0.68 g·kg^-1剂量组可以显著提高模型大鼠左后肢压痛痛阈、降低血清3种炎性因子含量(P<0.05,P<0.01)、改善神经根病变。结论: 腰痹通胶囊可以改善腰椎间盘突出动物模型的症状及神经根病变,其机制可能与抑制炎性因子释放有关。

Abstract:

Objective: To study the effect of Yaobitong capsule(YBT)on inflammatory cytokines and pain in the rat lumbar disc herniation model (RLDHM). Method: The model of lumbar disc herniation(LDH) was established by compressing silica gel pieces on the L₅ nerve root of rat. All the rats were randomized into model group,positive group(Yaotongning capsules,0.16 g·kg^-1)and YBT treatment groups(0.17,0.34,0.68 g·kg^-1). After administration for 30 d,the mechanical pain thresholds of left hind limb,serum tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6)levels and pathological change in the nerve root of rats were detected respectively. Result: The model of LDH in rats was successfully established. Compared with normal control group,the mechanical pain thresholds of the model rats were decreased significantly(P<0.05),the serum TNF-α,IL-1β,IL-6 levels were increased significantly(P<0.01),and the obvious lesion was observed in nerve root. In the 0.34, 0.68 g·kg^-1 dose of YBT groups,the mechanical pain thresholds were increased significantly,the serum TNF-α,IL-1β,IL-6 levels reduced significantly(P<0.05,P<0.01),and the pathological change was improved obviously in the nerve root. Conclusion: YBT could improve the symptoms and nerve root lesions in rats with LDH,which may be related with inhibition of inflammatory cytokines.