茶黄素对大鼠缺血性脑损伤的预防作用

Proventive Effects of Theaflavins on Ischemic Brain Injuries in Rats

摘要:

目的: 观察茶黄素对大鼠缺血性脑损伤的预防作用。方法: 64只健康成年SD大鼠随机分为4组:假手术组、模型组与茶黄素低、高剂量组。茶黄素低、高剂量组分别ig 20,40 mg·kg^-1茶黄素,假手术组和模型组分别ig等体积生理盐水。连续给药7 d后,采用大脑中动脉线栓法(MCAO)复制大鼠实验性脑缺血模型。缺血24 h后,观察大鼠行为并用Longa法进行神经行为学评分。从每组中随机取6只计算大鼠的脑组织含水量,其余10只测定脑组织超氧化物歧化酶(SOD),丙二醛(MDA),一氧化氮(NO)水平。结果: 与假手术组相比,模型组大鼠出现明显的行为学障碍,脑组织含水量,MDA,NO含量升高,SOD活性降低。与模型组相比,茶黄素低、高剂量组大鼠行为学评分显著提高,脑组织含水量和MDA含量显著降低,SOD活性显著提高,高剂量组NO含量显著降低。结论: 茶黄素对大鼠缺血性脑损伤有一定的预防作用。

Abstract:

Objective: To investigate the effects of theaflavin aganist ischemic brain injuries in rats. Method: Sixty-four SD rats were randomly divided into 4 groups as following:sham operation group (Sham), model group (Model), theaflavin low and high dose group, which was administrated with theaflavin 20, 40 mg·kg^-1 respectively. Rats of Sham and Model group were administrated with equal-volume normal saline. Rats were treated with continued 7 days, and then ischemic brain injuries in rats were reproduced by middle cerebral artery occlusion(MCAO). After operation 24 h, rats were observed behavior and neurological behavior score by Longa method. Then, water content of brain tissue in rats was calculated in 6 rats selected from each group. Finally, another 10 rats in each group were determined superoxide dismutase(SOD), malondialdehyde(MDA) and nitric oxide(NO) level in ischemic brain tissue by colorimetric methods. Result: Compared with Sham group, there were obvious behavioral disorders displayed in Model group, simultaneously water content of brain tissue, MDA and NO content were elevated, however, SOD activity was reduced. Compared with Model group, behavioral score and SOD activity in theaflavins groups were significantly ameliorated, yet water contents of brain tissue and MDA content were attenuated significantly. Besides, NO content was elevated significantly in theaflavins high dose group. Conclusion: Theaflavins can provent against ischemic brain injuries in rats.