不同配伍比例仙茅-淫羊藿药对的毒性与含量相关性

Correlation Between Toxicity and Content of Curculiginis Rhizoma and Epimedii Folium with Different Compatible Proportion

摘要:

目的: 比较探讨二仙药对仙茅-淫羊藿配伍的毒性来源、毒性与含量之间的相关性。方法: 在总量恒定的前提下,以仙茅-淫羊藿经典比例1:1为基准,向两侧扩展,均匀设计若干配伍方,以水提与醇提方式获得若干配伍方的提取物;将ICR小鼠随机分为10组,分别为水提和醇提仙茅-淫羊藿1:0,2:1,1:1,1:2,0:1组,给小鼠灌胃各配伍方提取物,比较各组毒性大小;用高效液相法测定各配伍方提取物中仙茅苷和淫羊藿苷的含量,分析其毒性与含量的相关性。结果: 小鼠急性毒性大小顺序为:(醇提)仙茅-淫羊藿(1:0)组 > (2:1)组 > (1:1)组 > (1:2)组 > (0:1)组 > 各水提组。仙茅苷含量与毒性呈显著性正相关(相关系数r=0.967,显著性检验P<0.01);淫羊藿苷含量与毒性呈显著不相关(相关系数r=-0.053,显著性检验P>0.05)。结论: 二仙药对配伍的毒性成分主要来自于仙茅且易富集于醇提物中,毒性大小与仙茅苷含量正相关。

Abstract:

Objective: To analyze the toxicity source of Curculiginis Rhizoma and Epimedii Folium and to study the correlation between their toxicity and the content of curculigoside and icariin with several compatible prescriptions. Method: Under the total dosage being constant, several compatible prescriptions were obtained by uniform design with Curculiginis Rhizoma and Epimedii Folium classical proportion 1: 1 as a reference. Their extracts were obtained being by water and alcohol. Their toxicity was compared in mice. The contents of curculigoside and icariin in each compatible prescriptions were determineby HPLC, and the correlation between toxicity and content were analyzed. Result: The acute toxicity sequence is alcohol extract of Curculiginis Rhizoma-Epimedii Folium(1: 0)>alcohol extract of Curculiginis Rhizoma-Epimedii Folium(2: 1)>alcohol extract of Curculiginis Rhizoma-Epimedii Folium (1: 1)>alcohol extract of Curculiginis Rhizoma-Epimedii Folium (1: 2)>alcohol extract of Curculiginis Rhizoma-Epimedii Folium (0: 1)>water extract group. There was significantly positive correlation between the content of curculigoside with their toxicity(r=0.967, P<0.01). No correlation was found between the content of Icariin and their toxicity (r=-0.053, P>0.05). Conclusion: Toxic components of Curculiginis Rhizoma and Epimedii Folium mainly come from Curculiginis Rhizoma , and they can be easily enriched in alcohol extracts. There is positive correlation between the toxicity and the content of curculigoside.