天山花楸叶黄酮类化合物在大鼠小肠的吸收特性

Intestinal Absorption Characteristic of Flavonoids from Sorbus tianschanica Leaves in Rats

摘要:

目的: 研究天山花楸叶总黄酮提取物在大鼠肠道的吸收部位及吸收机制. 方法: 运用在体单向肠灌流模型,采用HPLC测定灌流液中6种黄酮类成分的含量,检测波长360 nm,流动相乙腈-0.4%磷酸梯度洗脱,利用药物吸收速率常数(K_a)和药物表观吸收系数(P_app)分析天山花楸叶总黄酮提取物的吸收动力学特征. 结果: 天山花楸叶中6种黄酮类化合物在各肠段均有吸收,不同化合物在整个肠段的吸收程度存在差异,同一化合物在整个肠段的吸收无显著性差异.6种黄酮类化合物质量浓度20~80 mg·L^-1时, K_a和 P_app无显著性差异,药物吸收不受质量浓度的影响. 结论: 天山花楸叶黄酮类化合物在大鼠小肠的吸收机制为被动扩散,6种黄酮类化合物均不易于吸收.

Abstract:

Objective: To investigate intestinal absorption segments and mechanism of total flavonoids from Sorbus tianschanica leaves in rats. Method: Single-pass intestinal perfusion model was established in rats.The content of total flavonoids in perfusate was determined by HPLC with detection wavelength at 360 nm and mobile phase of acetonitrile and 0.4% phosphoric acid solution in gradient elution.Absorption rate constant (K_a) and apparent absorption coefficient (P_app) were evaluated absorption kinetics of total flavonoids. Result: Six kinds of flavonoids from S. tianschanica leaves could be absorbed in whole intestinal,extent of absorption existed differences in the entire intestinal among different compounds,no difference in absorption was observed in the same compound. K_a and P_app had no significant difference when concentrantions of six kinds of flavonoids was 20-80 mg·L^-1.The concentration of flavonoids had no effect on absorption kinetics. Conclusion: Absorption mechanism of six kinds of flavonoids from S. tianschanica leaves is passive diffusion,they are not easily absorbed in the intestine.