“冬病夏治”方药配伍对延胡索乙素经皮吸收特性的影响

Effect of “Treating Winter Disease in Summer” Prescription Compatibility on Percutaneous Absorption Properties of Tetrahydropalmatine

摘要:

目的: 探讨“冬病夏治”方药配伍对延胡索乙素经皮吸收特性的影响. 方法: 采用Franz扩散池和离体大鼠皮肤进行体外透皮试验,利用HPLC测定体外透皮接收液中延胡索乙素的含量,流动相甲醇-0.1%甲酸,检测波长280 nm,流速0.8 mL·min^-1.计算不同方药配伍的“冬病夏治”贴膏中延胡索乙素的累积渗透量和渗透速率. 结果: 不同方药配伍的“冬病夏治”贴膏中延胡索乙素体外透皮行为均符合零级动力学方程,全方组的透皮速率(1.04 μg·cm^-2·h^-1)和24 h累积渗透量(29.54 μg·cm^-2)均大于单味药,差异具有极显著性.全方组与缺甘遂和缺细辛组相比,延胡索乙素的体外渗透率无显著性差异,但与缺白芥子组相比具有显著性差异. 结论: “冬病夏治”方药配伍对延胡索乙素的经皮渗透特性有一定影响,方中白芥子能促进延胡索乙素的经皮吸收.

Abstract:

Objective: To investigate effect of “treating winter disease in summer” prescriptionon compatibility on percutaneous absorption of tetrahydropalmatine. Method: Franz-type diffusion cell was employed,taking isolated rat skin as transdermal barrier,HPLC was adopted to determine the content of tetrahydropalmatine in in vitro percutaneous absorption liquid with mobile phase of methanol-0.1% formic acid,detection wavelength at 280 nm and flow rate of 0.8 mL·min^-1.Cumulative permeation quantities and penetration rates of tetrahydropalmatine in “treating winter disease in summer” patches with different prescription compatibility were calculated. Result: In vitro percutaneous behavior of tetrahydropalmatine complied with the zero-order kinetics equation.Penetration rate (1.04 μg·cm^-2·h^-1) and cumulative permeation quantity (29.54 μg·cm^-2) of the whole group were greater than a single herb and difference was statistically significant.By comparing with the lack of Kansui Radix group and the lack of Asari Radix et Rhizoma group,effect of the whole group on in vitro percutaneous absorption of tetrahydropalmatine had no significant,however,compared with the lack of Semen Sinapis,difference was statistically significant. Conclusion: “Treating winter disease in summer” prescription compatibility has a certain influence on percutaneous absorption of tetrahydropalmatine,Semen Sinapis can enhance transdermal absorption of tetrahydropalmatine.