Box-Behnken设计-效应面法优化荆防止痒颗粒中挥发油提取工艺

Optimization of Extraction Process of Volatile Oil from Jingfang Zhiyang Granules by Box-Behnken Design Response Surface Methodology

摘要:

目的: 优化荆防止痒颗粒中挥发油的提取工艺,为该制剂的临床应用提供参考. 方法: 采用HPLC测定胡薄荷酮的含量,流动相甲醇-水(80 :20),检测波长252 nm.以挥发油提取量和挥发油中胡薄荷酮含量为指标,采用Box-Behnken设计-效应面法考察浸泡时间、提取时间及液料比对荆防止痒颗粒中挥发油提取工艺的影响. 结果: 最佳提取工艺为浸泡时间2 h,提取时间6.5 h,料液比9 mL·g^-1.挥发油提取量0.69 mL,胡薄荷酮质量浓度38.17 g·L^-1,与预测值的偏差较小. 结论: 优选的提取工艺合理可行,可有效保留荆防止痒颗粒中挥发性成分.

Abstract:

Objective: To optimize extraction process of volatile oil from Jingfang Zhiyang granules. Method: HPLC was employed to determine the content of pulegone with mobile phase of methanol-water and detection wavelength at 252 nm.Taking yield of volatile oil and the content of pulegone as indexes,Box-Behnken design-response surface methodology was adopted to optimize extraction process of volatile oil with soaking time,extraction time and solid-to-liquid ratio as factors. Result: Optimum extraction process was as follows:soaked 2 h and extracted 6.5 h with 9 times the amount of water.Yield of volatile oil was 0.69 mL with the content of pulegone was 38.17 g·L^-1,these values were similar to their predictive values. Conclusion: This optimized extraction process is reasonable and feasible,which can effectively retain volatile ingredients in Jingfang Zhiyang granules.