不同采收期栀子有机酸类成分HPLC指纹图谱

HPLC Fingerprint Analysis of Organic Acids in Gardeniae Fructus at Different Collecting Stages

摘要:

目的: 比较不同采收期栀子中有机酸类成分的HPLC指纹图谱,探讨该类成分在不同采收期内的动态变化. 方法: 采用Kromasil C₁₈色谱柱(4.6 mm×250 mm,5 μm),以0.05%三氟乙酸水溶液-乙腈为流动相梯度洗脱,流速1.0 mL·min^-1,检测波长327 nm,柱温40 ℃.运用相似度评价法对各样品指纹图谱进行化学模式识别研究. 结果: 以建立的栀子对照指纹图谱为基准,分别对不同采收期的样品图谱进行相似度评价,计算样品相似度为青栀子0.94~1.00,黄栀子0.81~0.92,红栀子0.53~0.74. 结论: 栀子中各有机酸类成分随采收期不同而各异,不同生长期内采集的样品中该类成分的积累具有一定规律.本研究所建立的方法可为栀子药材质量控制及实现规范化种植提供了科学参考.

Abstract:

Objective: To compare HPLC fingerprints of organic acids in Gardeniae Fructus at different collecting stages for determining the dynamic changes of these compounds. Method: The chromatographic separations were obtained on a Kromasil C₁₈ column (4.6 mm×250 mm, 5 μm) with a gradient elution using the mobile phase consisting of 0.05% trifluoroacetic acid aqueous solution and acetonitrile at a flow rate of 1.0 mL·min^-1. The detection wavelength was set at 327 nm, and the column temperature was 40 ℃. The HPLC fingerprints were analyzed by similarity evaluation analysis to conduct the chemical pattern recognition. Result: Using the established reference fingerprint of Gardeniae Fructus as standard, all sample fingerprints for green, yellow and red Gardeniae Fructus were evaluated, with the ranges of similarity 0.94-1.00,0.81-0.92 and 0.53-0.74, respectively. Conclusion: The study offers the basis of quality control of Gardeniae Fructus and the realization of good agricultural practice.