α-细辛脑原位凝胶体外分析方法的建立

Analysis Method of Alpha Asarone in Situ Gel in Vitro

摘要:

目的: 建立 α-细辛脑原位凝胶含量测定和体外释放度测定方法. 方法: 采用HPLC法, SHIM-PACK VP-ODS色谱柱(4.6 mm×250 mm,5 μm),流动相甲醇-水(75:25),检测波长258 nm,采用磷酸盐缓冲溶液(pH 7.4)作为释放液. 结果: 含量测定中 α-细辛脑在10.4~104 mg·L^-1与峰面积具有良好的线性关系(r=1),平均回收率100.6%,RSD 2.2%.体外释放度试验中 α-细辛脑在2.06~82.4 mg·L^-1线性较好(r=0.999 9),平均回收率95.83%,RSD 2.5%. 结论: 建立的分析方法具有专属性好、准确和可靠的特点,可为后续研究奠定基础.

Abstract:

Objective: Set up the analysis method of alpha asarone in situ gel and the analysis method of it's in vitro release. Method: HPLC instrument with SHIM-PACK VP-ODS column(4.6 mm×250 mm,5 μm)was used.The mobile phase consisted of methanol-water (75 : 25),The wavelength for detection was 258 nm. And using pH 7.4 slightly PBS as release liquid. Result: The liner range of alpha asarone was 10.4-104 mg·L^-1(r=1).The average recovery was 100.6%,RSD was 2.19% in the determination of experiment.The liner range of alpha asarone was 2.06-82.4 mg·L^-1(r=0.999 9).The average recovery was 95.83%,RSD was 2.5% in the in vitro release experiment. Conclusion: The method is exclusive,accurate and reliable.And it can lay the foundation for follow-up study.