薄荷-荆芥穗药对挥发油成分的GC-MS分析

Chemical Constituents of Volatile Oils in Drug Pair of Menthae Haplocalycis Herba and Schizonepetae Spica by GC-MS

摘要:

目的: 考察薄荷-荆芥穗配伍前后挥发油成分的变化,为薄荷-荆芥穗药对有效物质基础研究提供依据. 方法: 采用水蒸气蒸馏法提取了薄荷-荆芥穗药对、薄荷和荆芥穗单味药材的挥发油,通过GC-MS分析方法对二者配伍前后挥发油成分进行分析. 结果: 薄荷挥发油中确定了40个化合物,其中21个在药对挥发油中未检测到;荆芥穗挥发油中检测确定了27个化合物,其中6个在药对挥发油中未检测到;药对挥发油中确定了35个化合物,其中有5个新成分在单味药材挥发油中未检测到. 结论: 薄荷-荆芥穗药对挥发油的化学成分在组成及相对含量较单味药材均有一定变化,这些变化可能是煎煮过程中发生了某些物理或化学的变化.配伍后化合物组群的相应药效学还值得深入研究.

Abstract:

Objective: To discuss the influence of drug compatibility on the components of the volatile oil in Menthae Haplocalycis Herba and Schizonepetae Spica, and to provide a basis for researching basis of effective material for the drug pair. Method: Steam distillation was used to extraction of the volatile oil Menthae Haplocalycis Herba,Schizonepetae Spica and their combination. GC-MS was used to analyze the volatile oil of them. Result: 40 compounds were identified from the volatile oil of Menthae Haplocalycis Herba, including 21 compounds were not detected from the drug pair oil;27 compounds were identified from the volatile oil of Schizonepetae Spica, including 6 compounds were not detected from the drug pair oil. In volatile oil of the drug pair, there are 35 compounds were identified, including 5 new components that were not detected in volatile oil of single Medicinal herbs. Conclusion: There were some changes between the volatile oil of single Medicinal herbs and the volatile oil of the drug pair.These phenomena may be due to the occurrence of certain chemical reactions and physical changes in compatibility of decocting process. Further research is worth change compound group and the corresponding compatibility after decocting efficacy.