HS-SPME-GC-MS联用分析稳心颗粒中的挥发性成分

Analysis of Volatile Components of Wenxin Granule by HS-SPME-GC-MS Technique

摘要:

目的: 对稳心颗粒进行挥发性成分的快速分析,明确其主成分及相对含量. 方法: 采用顶空固相微萃取(HS-SPME)与气相色谱-质谱(GC-MS)联用技术对稳心颗粒中的挥发性成分进行提取及快速分析,检索NIST08.L质谱库(匹配度 ≥80%),并结合文献报道鉴定了其中的化学成分. 结果: 从稳心颗粒挥发性成分中共鉴定出52种化合物,占总挥发性成分的67.39%,其中匙叶桉油烯醇、异丁香烯、氧化丁香烯、香橙烯等8个化合物为其主要成分,与文献报道的甘松挥发油主成分基本一致. 结论: 通过对其挥发性成分进行分析,为进一步研究稳心颗粒的药效物质基础提供了科学依据,并为其质量控制研究打下实验基础.

Abstract:

Objective: To study the chemical components of the volatile oil from Wenxin granule including the main constituents and their relative content percentages among total oil. Method: The chemical components of the volatile oil from Wenxin granule were extracted and analyzed by applying Head Space Solid Phase Micro Extraction (HS-SPME) and GC-MS techniques. The chemical components were identified by retrieving NIST08.L mass spectral library (matching ≥80%), combined with the reported literature data. Result: The 52 components were identified from the volatile oil of Wenxin granule, accounting for 67.39%. Among which, spathulenol, isocaryophyllene, caryophyllene oxide and other five compounds were the main constituents. The result was basically identical with the data reported in the literatures of the volatile oil of Nardostachyos Radix et Rhizoma. Conclusion: The research provides basis for the further material basis study of the Wenxin granule, and accumulates laboratory data for optimization of quality control.