HPLC同时测定不同产地猴耳环叶中没食子酸和槲皮素的含量

HPLC Determination of Gallic Acid and Quercetin in Archidendri Folium from Different Producing Areas

摘要:

目的: 建立HPLC同时测定不同产地猴耳环叶中没食子酸和槲皮素含量的方法. 方法: 采用Odyssil C₁₈色谱柱(4.6 mm×250 mm,5 μm),流动相乙腈(A)-0.2%磷酸水溶液(B)进行梯度洗脱,流速1.0 mL·min^-1,柱温,30 ℃,检测波长254 nm;进样量5 μL. 结果: 没食子酸在0.060 2～1.806 μg线性良好(r=0.999 5),平均回收率为97.27%,RSD 1.4%;槲皮素在0.016 9～0.507 6 μg线性良好(r=0.999 6),平均回收率为96.29%,RSD 1.3%. 结论: 该方法简便可行、重复性好,适用于猴耳环药材2种有效成分的同时测定,可为猴耳环药材的质量控制提供参考依据.

Abstract:

Objective: To establish a determination method for gallic acid and quercetin in Archidendri Folium from different producing areas. Method: HPLC was performed on Odyssil C₁₈ column (4.6 mm×250 mm, 5 μm), gradiently eluting with acetonitrile as mobile phase A and 0.2% H₃PO₄ as B at a flow rate of 1.0 mL·min^-1. The column temperature was 30 ℃. The detection wavelength was 254 nm. The injection volume was 5 μL. Result: The linearity of gallic acid was good in the range of 0.060 2-1.806 μg (r=0.999 5), and the average recovery was 97.27% (RSD 1.4%). The linearity of quercetin was good in the range of 0.016 9-0.507 6 μg (r=0.999 6), and the average recovery of 96.29%(RSD 1.3%). Conclusion: The established method is simple, accurate and can be used for the determination of gallic acid and quercetin in Archidendri Folium, it can able to provide reference for quality control of Archidendri Folium.