毛鸡骨草中总黄酮含量测定方法

Determination of Total Flavonoids in Abrus mollis

摘要:

目的: 建立毛鸡骨草总黄酮含量的专属性测定方法. 方法: 采用紫外-可见分光光度法研究三乙胺显色剂及其不同含水乙醇溶液对黄酮类成分光谱吸收变化影响. 结果: 不同黄酮类显色后光谱吸收峰变化存在明显差异;三乙胺法适应于芹菜素黄酮类含量测定,且不同体积分数乙醇对其吸收光谱具有显著影响,当加入50%乙醇显色后光谱吸收值稳定在400 nm,经方法学考察,芹菜素-6-C-葡萄糖-8-C-葡萄糖苷对照品在0.507～101.4 mg·L^-1与吸光度具有良好的线性关系(r=0.999 5),平均加样回收率为98.01%,RSD 2.1%. 结论: 毛鸡骨草总黄酮含量的测定可采用三乙胺-50%乙醇显色法,该方法对于芹菜素母核黄酮类具有良好的专属性和准确性,操作简单易行.

Abstract:

Objective: To establish an exclusive method to determine the content of total flavonoids in Abrus mollis. Method: UV-VIS spectrophotometry was used to study the absorption spectrum changes of flavonoids in triethylamine (TEA) chromogenic agent with different concentrations of ethanol aqueous solution medium. Result: There was obvious difference between different flavonoids before and after coloration. The TEA method could be used to determine the content of apigenin flavonoids and and their absorption spectra were remarkably influenced by different concentrations of ethanol aqueous solution with the stable absorption wavelength of 400 nm after coloration in 50% ethanol aqueous solution. After the methodological study, 6-C-glycopyranosyl-8-C-glycopyranosyl apigenin had a good linearity in the ringe of 0.507-101.4 mg·L^-1(r=0.999 5), the average recovery was 98.01%with RSD of 2.1%. Conclusion: The content of total apigenin flavonoids in A. mollis can be determined by the TEA method in 50% EtOH aqueous solution with good specificity, accuracy and simple operation.