加味龙牡宁神汤对抑郁症小鼠模型的影响

Effects of Modified Longmu Ningshen Decoction on Mouse Model of Depression

摘要:

目的: 以抑郁症小鼠为模型,观察加味龙牡宁神汤对抑郁症小鼠的影响. 方法: 取ICR小鼠60只,随机分为5组,分别为正常组、氟西汀组(25 mg·kg^-1)、加味龙牡宁神汤4,8,16 g·kg^-1组,每组均按0.02 mL·g^-1体重给小鼠 ig,其中正常组灌服等量生理盐水,每日1次,连续给药10 d,通过检测小鼠体重、行为变化,观察加味龙牡宁神汤对小鼠急性应激的影响;另取小鼠72只,随机分为6组,分别为正常组、利血平模型组、氟西汀组(25 mg·kg^-1)、加味龙牡宁神汤4,8,16 g·kg^-1组,其中正常组、利血平模型组 ig等量生理盐水,每日 ig 给药1次,连续给药10 d,观察小鼠眼睑不能、出圈个数、体温,采集小鼠血清和全脑测定白细胞介素-2(IL-2),5-羟色胺(5-HT)含量,观察加味龙牡宁神汤对抑郁症小鼠的影响. 结果: 加味龙牡宁神汤对急性应激反应的结果显示,与正常组比较,加味龙牡宁神汤能明显缩短小鼠游泳和悬尾累积不动时间(P <0.05, P <0.01);加味龙牡宁神汤对抑郁症小鼠的结果显示,与模型组比较,加味龙牡宁神汤能明显降低血清中IL-2水平,明显拮抗利血平导致的小鼠体温下降、眼睑下垂及活动不能状态和利血平引起的5-HT含量下降(P <0.05, P <0.01). 结论: 加味龙牡宁神汤有一定的抗抑郁作用,其作用机制可能与增加5-HT含量和降低IL-2水平有关.

Abstract:

Objective: To observe the effects of Modified Longmu Ningshen decoction (MLND) on the mouse model of depression. Method: Sixty ICR mice were randomly divided into 5 groups:the normal group (normal saline), the fluoxetine group (25 mg·kg^-1) and the low-, medium-, high-dose MLND groups (4, 8, 16 g·kg^-1). The corresponding medicines and normal saline at 0.02 mL·kg^-1 body weight were administrated to the mice once daily for 10 days. The effect on acute stress including tail suspension immobility time and swimming immobility time, the changes of body weight, body temperature and behavior were observed. Another 72 ICR mice were randomly divided into 6 groups:the normal group (normal saline), the model group (reserpine), the fluoxetine group (25 mg·kg^-1) and the low-, medium-, high-dose MLND groups (4, 8, 16 g·kg^-1). The corresponding medicines at 0.02 mL·kg^-1 body weight were administrated to the mice once daily for 10 days. The effect on depression including ptosis, akinesia and temperature were observed. The contents of interleukin-2 (IL-2) and 5-hydroxytryptamine (5-HT) in serum and the whole brain were determined. Result: Compared with the normal group, MLND could shorten the immobility time of tail suspension and swimming of mice in the acute stress experiment (P <0.05, P <0.01). MLND could reduce the levels of IL-2, inhibit the reserpine-induced body temperature rising, eye ptosis and akinesia of mice, and decrease the level of 5-HT significantly (P <0.05, P <0.01). Conclusion: MLND has a certain anti-depression effect and the mechanism may be related to increasing 5-HT and decreasing of IL-2.