左归丸对GDM大鼠胎盘组织11 β-HSD1表达的影响

Effects Zuogui Wan on mRNA Expression of 11 β-HSD1 in Placenta of Gestational Diabetes Mellitus Rats

摘要:

目的: 探讨左归丸对妊娠糖尿病大鼠胎盘组织11 β-羟基类固醇脱氢酶1(11 β-HSD1)基因表达水平的影响. 方法: 育龄雌性SD大鼠受孕后随机分为正常妊娠组(C)、妊娠糖尿组(GDM)、胰岛素干预组(INS)、左归丸干预组(ZGW)、左归丸联合胰岛素干预组(F),每组10只;受孕当日GDM组和3个干预组按45 mg·kg^-1 ip链脲佐菌素(STZ)复制妊娠糖尿病模型.并于孕4 d开始给药干预(左归丸10 g·kg^-1 ig,胰岛素20 U·kg^-1 ih).孕19 d断头取血,葡萄糖氧化酶法测定血糖;酶联免疫吸附法检测血清皮质酮、胰岛素;采用RT-qPCR技术检测胎盘11 β-HSD1基因mRNA的表达水平,并进行定量分析. 结果: 与正常妊娠组比较,GDM组血糖水平、血清胰岛素浓度、皮质酮水平、胎盘11 β-HSD1基因mRNA的表达水平均明显升高,差异有统计学意义(P <0.01);与妊娠糖尿组比较,左归丸干预组血糖水平、血清胰岛素浓度、皮质酮水平、胎盘11 β-HSD1基因mRNA的表达水平均明显降低(P <0.05, P <0.01),但仍高于正常妊娠组,联合组治疗效果优于左归丸组. 结论: 左归丸可降低妊娠期糖尿病大鼠血糖水平,胎盘部位有11 β-HSD1基因的表达,妊娠糖尿病病理状态可影响11 β-HSD1基因在胎盘的表达水平,左归丸有下调11 β-HSD1基因表达水平的作用,推测可能是其改善GDM大鼠胰岛素抵抗的机制之一.

Abstract:

Objective: To study the effects of Zuogui Wan on the mRNA expression of 11 β-hydroxysteroid dehydrogenase type 1(11 β-HSD1) in placenta of gestational diabetes mellitus (GDM) rats. Method: Pregnant reproduction age SD rats were randomly divided into 5 groups:the normal pregnancy (C) group, the GDM control (GDM) group, the diabetes insulin treated (INS) group, Zuogui Wan treated (ZGW) group and Zuogui Wan combined insulin treated (F) group. The GDM was induced except the C group by injecting streptozocin (STZ) abdominally at 45 mg·kg^-1. The rats in the three treated groups received the treatment of Zuogui Wan and/or insulin from injecting STZ until 19-day pregnant. Glycemia was detected by using the glucose oxidase method. The corticosterone and insulin in blood serum were detected by using ELISA kit. The mRNA expression level of 11 β-HSD1 in placenta was evaluated by RT-qPCR, and the quantitative analysis was conducted. Result: Compared with the C group, the glycemia, serum insulin, corticosterone level and placental 11 β-HSD1 mRNA expression level increased in the GDM group (P <0.01). Compared with the GDM group, the glycemia level, serum insulin, corticosterone level and placental 11 β-HSD1 mRNA expression level decreased significantly in AGW and F groups, but the results were higher than those in the C group. Moreover, the effect is better in F group. Conclusion: Zuogui Wan could reduce the level of glycemia in diabetic rats. The mRNA expression of 11 β-HSD1 was found in placental and could be influenced by GDM. Zuogui Wans have the improving effects on the glycemic status of GDM rats, which may be related to reducing the placental 11 β-HSD1 gene expression level.