肉蔻-5味丸对慢性抑郁模型大鼠行为及海马单胺递质的影响

Experimental Research on Nutmeg Wuwei Pills Against of Depression Model Rat Behavior and Hippocampus Monoamine Neurotransmitters

摘要:

目的: 观察蒙药肉蔻-5味丸对慢性抑郁模型大鼠行为及海马单胺递质的影响,探讨其抗抑郁作用机制. 方法: 60只雄性SD大鼠,随机分为正常组、模型组、盐酸氟西汀组(3.6 mg·kg^-1)及肉蔻-5味丸低、中、高剂量组(0.2,0.4,0.8 g·kg^-1),每组10只.采用慢性不可预见性应激与孤养结合的造模方法复制抑郁模型,造模同时 ig给药,每天1次,连续给药21 d.以体重变化,矿场试验(open-field test),糖水消耗试验来进行行为学评价,酶联免疫法(ELISA) 检测大鼠海马5-羟色胺(5-HT),去甲肾上腺素(NE),多巴胺(DA) 单胺递质的含量. 结果: 给予21 d应激后,模型组大鼠体重增长缓慢,糖水消耗量、Open-field试验水平活动得分和垂直活动得分及海马5-HT,DA,NE的含量较正常组显著降低(P <0.05, P <0.01),与模型组相比氟西汀和肉蔻-5味丸可不同程度改善其抑郁状态,氟西汀组、肉蔻-5味丸高、中剂量组大鼠海马5-HT,DA,NE的含量显著增加(P <0.05, P <0.01). 结论: 肉蔻-5味丸的抗抑郁作用可能与提高脑内5-HT,NE,DA单胺递质的含量相关.

Abstract:

Objective: To observe the effect of Nutmeg Wuwei pills on the depressive behavior and the monoamine neurotransmitters of depression rat model andexplore the mechanism. Method: Sixty male SD rats were randomly divided into normal group, model group, fluoxetine group(3.6 mg·kg^-1) and Nutmeg Wuwei pills low, medium and high dose groups(0.2,0.4,0.8 g·kg^-1),10 rats in each group. After 21 days, behavior evaluation was made through observing the rats weight, open-field experiment and sugar consumption experiment. Enzyme linked immunosorbent assay(ELISA) method was used for determination of the content of the rat hippocampus 5-hydroxytryptamine(5-HT), norepinephrine(NE) and dobaamine(DA). Result: Compared with normal group, the scores of horizontal activity and vertical activity, sugar consumption were decreased significantly in the model group. Compared with model group, fluoxetine group and Nutmeg Wuwei pills groups got significantly higher scores of horizontal activity, vertical activity and sugar consumption. Nutmeg Wuwei pills could increase hippocampus 5-HT, NE and DA content, especially which was significant in the medium and high dose groups (P <0.05, P <0.01). Conclusion: Nutmeg Wuwei pills can improve abnormal behavior of depressive model rats and improve the neurotransmitter NE, 5-HT, DA. The anti-depressive mechanism is mainly due to increasing the cerebral monoamine neurotransmitters concentration in the brains.