益智仁盐炙前后分别组成缩泉丸的缩尿部位指纹图谱比较

Comparison on Fingerprint Spectrum of Urine-reducing Part of Suoquan Wan Constituted with Non-processed or Salt-processed Alpiniae Oxyphyllae Fructus Fruits

摘要:

目的：通过比较益智仁盐炙前后组成缩泉丸缩尿部位的指纹图谱，从物质基础上揭示益智仁盐炙后增强缩泉丸缩尿药效的原因。方法：采用HPLC，流动相乙腈-水梯度洗脱，检测波长230 nm。记录10批益智仁盐炙前后分别组成缩泉丸的石油醚部位HPLC图谱，建立相应指纹图谱，标定特征共有峰并比较其平均相对峰面积和平均相对保留时间。结果：益智仁盐炙后组成缩泉丸时，有11个共有峰平均相对面积明显增加，包括对缩尿药效贡献较大的1号，4号（teuhetenone A），5号（7-表-香科酮），7号色谱峰，增加率分别为123.08%，90.91%，53.33%，43.75%。结论：对缩尿作用贡献较大的4个成分含量的明显增加极有可能与缩泉丸缩尿作用的增强紧密相关，此外其他7个成分含量也有明显增加。

Abstract:

Objective: A comparison was made between fingerprint spectrum of urine-reducing part of Suoquan Wan constituted with non-processed and salt-processed Alpiniae Oxyphyllae Fructus fruits to discuss the chemical composition changes after salt-processing that enhance the urine-reducing effect. Method: HPLC was used for analysis with mobile phase of acetonitrile-water for gradient elution and detection wavelength at 230 nm.HPLC spectra of 10 batches Suoquan Wan constituted with non-processed and salt-processed Alpiniae Oxyphyllae Fructus fruits were recorded.Establishing the fingerprint spectra,32 peaks marked on each fingerprint spectrum had been compared. Result: The average relative area of 11 common peaks increased after salt-processing of Alpiniae Oxyphyllae Fructus fruits.Chromatographic peaks of No.1,No.4 (teuhetenone A),No.5 (7-epi-teucrenone B) and No.7 were the major contribution to urine-reducing effect,which increased 123.08%,90.91%,53.33% and 43.75%,respectively. Conclusion: The change of those 4 components maybe closely related to the enhancement of urine-reducing effect of Suoquan Wan,and the change of other 7 components may be synergistic to it.