神曲鲜干品组方对食积小鼠胃肠动力及肠道菌群调整的影响

Effect of Massa Medicata Fermentata Composed by Fresh or Dry Chinese Medicines on Gastrointestinal Motility and Intestinal Flora Regulating of Dyspepsia Mice

摘要:

目的：探讨神曲中青蒿等鲜干品组方及不同制法对食积小鼠胃肠动力及肠道菌群调整的影响，为优化神曲组方并揭示其作用机制提供依据。方法：制备4组神曲样品，分别为基本组（面粉、赤小豆、苦杏仁），鲜品组（鲜品榨汁、煎汁）和干品组（干品煎汁）。采用昆明种小鼠按0.4 mL·g^-1灌胃100%精炼猪脂制备食积小鼠胃肠动力障碍模型，每日1次，连续10 d。动物分为6组（空白组、模型组和4组神曲样品）。样品组按0.2 mL·g^-1灌胃给予相应神曲混悬液，空白组和模型组灌胃等量水，每日1次，连续7 d。观察各组样品对食积小鼠胃排空率、小肠推进率的影响。采用聚合酶链式反应技术检测各组小鼠结肠内容物菌群状况。结果：与模型组比较，鲜干品各组对胃排空及肠推进均有一定促进作用，但程度不同。其中鲜品煎汁组能显著提高胃排空及肠推进作用；鲜品榨汁组能显著提高肠推进作用。干品组对胃排空及肠推进均有一定影响，但差异不具有显著性。基本组仅对胃排空有显著促进作用，其肠推进作用在各组中最弱。与模型组比较，各给药组均可增加肠道乳酸杆菌、双歧杆菌、拟杆菌的量，但影响程度不同，排序为鲜品煎汁组 > 鲜品榨汁组 > 干品煎汁组 > 基本组。结论：神曲中青蒿等鲜品组方入药可促进食积小鼠胃肠推动作用及肠道菌群调整，两者间作用协同一致。提示鲜品入药优于干品，尤以青蒿等鲜品煎汁入药为佳；神曲健脾消食作用与促进胃肠动力、调整肠道菌群并促进有益菌生长有关。

Abstract:

Objective: To discuss the effect of Massa Medicata Fermentata composed by fresh or dry Chinese medicines and prepared by different preparation methods on gastric emptying,intestine propulsion and regulating intestinal flora of dyspepsia mice. Method: Four groups of Massa Medicata Fermentata samples were prepared,the first group is the basic one composed of flour,rice bean and bitter almond,the fresh product groups (the basic group and fresh medicine juicing or decocting) and the dry product group (the basic group and dry medicine decocting).Mice were used to set up dyspepsia and gastrointestinal disorders model by ig 100% refine lard oil with dose of 0.4 mL·g^-1 for 10 d.Experimental animals were divided into 6 groups (the blank group,the model group and four sample groups).Sample groups were given Massa Medicata Fermentata suspension by oral administration with 0.2 mL·g^-1,the blank group and the model group were administrated equivalent distilled water,once a day and for seven consecutive days.Impact of sample groups on gastric emptying rate and intestine propulsion rate was observed.Flora condition in colonic contents was detected by polymerase chain reaction technique (PCR). Result: In the gastrointestinal dynamics experiment,compared with the model group,each drug administration group could promote the movement of gastric emptying and intestinal propulsion,but their levels were different.In the detection of intestinal flora,each drug administration group could raise significantly the growth of intestinal beneficial bacteria (such as lactobacillus,bifidobacterium and bacteroides) and their levels were different. Conclusion: Massa Medicata Fermentata composed of fresh Chinese medicines can promote stomach intestine movement significantly and regulate the intestinal flora,both experiments led to corresponding results together.Massa Medicata Fermentata composed of fresh Chinese medicines is better than dry ones.The principles of formulating prescription about Massa Medicata Fermentata is fresh medicine decocting for the best.The spleen function of Massa Medicata Fermentata is concerned with promoting gastrointestinal,adjusting the intestinal flora and promoting the growth of bacteria.