熟地黄炭补血止血作用的炮制机制探索

Dicussion on Processing Mechanism of Charred Rehmanniae Radix Praeparata with Nourishing Blood and Hemostatic Effect

摘要:

目的：筛选熟地黄炭止血的主要药效部位，探讨其补血止血作用的炮制机制。方法：以小鼠的出血时间（BT）和凝血时间（CT）为指标筛选熟地黄炭止血的有效部位；以SD大鼠为研究对象，通过腹腔注射环磷酰胺同时结合尾部放血，以8%无水乙醇代替自由饮水的方法共同建立大鼠血虚出血模型，观察其一般体征、病理组织，测定大鼠的血常规指标即红细胞总数（RBC），血红蛋白含量（HGB）和白细胞总数（WBC）；检测血液流变学指标（高、中、低切下全血还原黏度及血浆黏度）和凝血4项指标[凝血酶原时间（PT），凝血酶时间（TT），活化部分凝血活酶时间（APTT）和血浆纤维蛋白原（FIB）]。结果：与空白组比较，熟地黄炭组、熟地黄炭水部位组均可缩短小鼠BT和CT。与空白组比较，血虚出血模型组大鼠的RBC，HGB和WBC均明显降低；高、低切下的全血还原黏度升高；PT，TT和APTT均延长，FIB增多。与模型组比较，熟地黄炭组大鼠的RBC，HGB和WBC均明显升高；高、中、低切下的全血还原黏度及血浆黏度均降低；PT，TT和APTT均缩短，FIB减少。与模型组比较，熟地黄炭水部位组大鼠的RBC和HGB均升高；高切下的全血还原黏度降低；PT，TT和APTT均缩短，FIB减少。结论：熟地黄炭通过升高大鼠RBC，提高HGB，改善环磷酰胺与放血所致血虚大鼠的体征，表现了其补血的作用；同时通过缩短大鼠TT，PT和APTT，降低FIB的含量，表现了其止血的作用。提示熟地黄炭针对血虚出血大鼠模型发挥了较好的补血止血作用。

Abstract:

Objective: To dicuss processing mechanism of charred Rehmanniae Radix Praeparata with nourishing blood and hemostatic effect by screening its main active site. Method: Effective parts of charred Rehmanniae Radix Praeparata for hemostatic efficacy were screened with bleeding time (BT) and clotting time (CT) as indexes.Taking SD rats as the research object,by intraperitoneal injection of cyclophosphamide combined with tail bloodletting,and with 8% ethanol instead of free drinking water to establish rat model with blood deficiency and bleeding,the general physical signs and pathological tissue were observed,red blood cell count (RBC),hemoglobin content (HGB) and white blood cell count (WBC) were determined;hemorrheological parameters and blood clotting tetrachoric[prothrombin time (PT),thrombin time (TT),activated partial thromboplastin time (APTT) and plasma fibrinogen (FIB)] were measured. Result: Compared with the blank group,charred Rehmanniae Radix Praeparata and its water part could shorten BT and CT of mice.Compared with the blank group,RBC,HGB and WBC of blood deficiency and bleeding model group rats decreased significantly;whole blood reduced viscosity under high and low shear increased;PT,TT and APTT prolonged and FIB increased.Compared with the model group,RBC,HGB and WBC of rats in the charred Rehmanniae Radix Praeparata group increased significantly;whole blood reducted viscosity and plasma viscosity under high,medium and low shear were decreased;PT,TT and APTT were shortened and FIB was reduced.Compared with the model group,RBC and HGB of rats in water part group increased;whole blood reduced viscosity under high shear was decreased;PT,TT and APTT were shortened and FIB was reduced. Conclusion: Charred Rehmanniae Radix Praeparata has a good nourishing blood and hemostatic effect for rat model with blood deficiency and bleeding.