响应面优化黄秋葵叶多糖的提取工艺及其抗氧化活性考察

Response Surface Methodology for Optimization of Extraction and Antioxidant Activities of Polysaccharides from Abelmoschus esculentus Leaves

摘要:

目的：优化黄秋葵叶多糖的提取工艺条件，并对其体外抗氧化活性进行评价，为黄秋葵叶的开发提供参考。方法：在单因素试验基础上，选择超声波功率、提取温度和提取时间为考察因素，应用Box-Behnken中心组合进行三因素三水平设计，以多糖提取率为响应值，采用响应面法优化黄秋葵叶多糖的提取工艺。通过DPPH·和·OH的清除能力考察黄秋葵叶多糖的体外抗氧化活性。结果：黄秋葵叶多糖的最佳提取工艺条件为超声功率160 W，温度54℃，时间91 min；黄秋葵叶多糖的提取率为4.81%，与理论值偏差很小。黄秋葵叶多糖清除DPPH·和·OH的半抑制浓度（IC₅₀）分别为2.65，1.47 g·L^-1。结论：优选的提取工艺稳定可行、提取率高，黄秋葵叶多糖具有较好的抗氧化活性，为黄秋葵的工业化生产提供参考。

Abstract:

Objective: To optimize extraction process of polysaccharides from Abelmoschus esculentus leaves by response surface methodology and investigate its in vitro antioxidant activity, in order to provide reference for developing A.esculentus leaves. Method: Based on single-factor experiments, three independent factors, namely ultrasonic power, extraction temperature and extraction time, were selected as affecting factors for Box-Behnken central composite experiment design. With the polysaccharide extraction rate as the response value, the response surface analysis method was applied to optimize the polysaccharide extraction technique of A.esculentus leaves. DPPH·and·OH scavenging assays were carried out to study in vitro antioxidant activity of polysaccharides from A.esculentus leaves. Result: The results showed that the optimum conditions for extracting polysaccharides from A.esculentus leaves were ultrasonic power at 160 W, extraction temperature at 54℃, extraction time of 91 min. Under these conditions, the extraction rate of polysaccharides was 4.81%, with a small deviation from the theoretical value. The half inhibiting concentrations (IC₅₀) to DPPH·and·OH were 2.65, 1.47 g·L^-1 respectively. Conclusion: The optimized extraction technique is stable and feasible, with a high extraction rate. Polysaccharides from A.esculentus leaves have a better anti-oxidation activity, and provide reference for industrialized production of A.esculentus.