HPLC同时测定白花蛇舌草注射液中5种成分的含量

Simultaneous Determination of Five Compounds in Hedyotis diffusa Injection by HPLC

摘要:

目的：利用HPLC同时测定白花蛇舌草注射液中5种成分的含量。这5种成分包括环烯醚萜苷类（10-乙酰鸡矢藤苷、鸡矢藤苷甲酯、去乙酰车叶草苷酸甲酯）和黄酮苷类{槲皮素-3-O-[2-O-（6-O-E-阿魏酰基）-D-吡喃葡糖基]-β-D-吡喃半乳糖苷、山柰酚-3-O-[2-O-（6-O-E-阿魏酰基）-β-D-吡喃葡糖基]-β-D-吡喃半乳糖苷}。方法：采用SunFire^TM C₁₈色谱柱（4.6 mm×250 mm，5 μm），以乙腈-水（含0.1%甲酸）为流动相，梯度洗脱，柱温30℃，流速1.0 mL·min^-1。结果：各对照品在线性范围内线性关系良好，相关系数均≥0.999 5；加样回收率为98.1%~101.2%，RSD均≤3.3%。结论：该方法操作简便，结果可靠，重复性好，可作为白花蛇舌草注射液质量评价的参考依据。

Abstract:

Objective: To develop an HPLC method for simultaneous determination of five compounds, including three iridoid glucosides (10-acetyl-scandoside, scandoside methyl ester, and deacetylasperuloside methyl ester) and two flavonoid glucosides {quercetin-3-O-[2-O-(6-O-E-ferloyl)-β-D-glucopyranosyl]-β-D-galactopyranoside and kaempferol-3-O-[2-O-(6-O-E-ferloyl)-β-D-glucopyranosyl]-β-D-galactopyranoside} in Hedyotis diffusa injection. Method: The separation of targeted compounds was performed on SunFire^TM C₁₈ column (4.6 mm×250 mm, 5 μm) with acetonitrile-0.1% formic acid solution as the mobile phase for gradient elution. The column temperature was 30℃ and the flow rate was 1.0 mL·min^-1. Result: The method showed good linearity (r>0.999 5) within the linear range. The recoveries were in the range of 98.1%-101.2% with RSD<3.3%. Conclusion: The method is reliable, simple and reproducible, and it can be used to control the quality of Hedyotis diffusa injection.