胡颓子叶药材HPLC指纹图谱

HPLC Fingerprint of Elaeagnus pungens Leaves

摘要:

目的：建立了胡颓子叶药材的指纹图谱分析方法，为胡颓子叶药材质量控制提供新的方法。方法：采用高效液相色谱法，色谱条件为Agilent ZOTBAX XDB-PhenylC₁₈色谱柱（4.6 mm×250 mm，5 μm），流动相0.1%磷酸水-乙腈梯度洗脱，柱温30℃，流速1.0 mL·min^-1，检测波长360 nm。运用国家药典委员会“中药色谱指纹图谱相似度评价系统软件A版”对12批胡颓子叶药材进行相似度评价。结果：以银锻苷为参照峰，确定了16个共有峰构成12批胡颓子叶药材的指纹特征指纹图谱，各批样品的相似度均>0.8。并采用欧氏距离平方法进行聚类分析。结论：该方法准确稳定，重复性好，可为胡颓子叶药材的质量控制提供依据。

Abstract:

Objective: To establish an HPLC-fingerprint (HPLC-FP) analysis method for Elaeagnus pungens leaves and provide a new method for their quality control. Method: HPLC separation was performed on Agilent ZOTBAX XDB-Phenyl C₁₈(4.6 mm×250 mm, 5 μm) with 0.1% phosphoric acid-acetonitrile as the mobile phase for gradient elution; the column temperature was 30℃; the flow rate was 1.0 mL·min^-1; the detection wavelength was set at 360 nm. The Similarity Evaluation System for Chromatographic Fingerprint of TCM (2004A edition) was used to evaluate the similarity of 12 batches of E. pungens leaves. Result: With tiliroside as the reference peak, sixteen common peaks were obtained in 12 batches of samples. The similarity of each fingerprint within one group was over 0.80, and squared Euclidean distance method was used for clustering analysis. Conclusion: This method shows high precision, stability and good reproducibility, and it can provide basis for the quality control of E. pungens leaves.