洪连对ANIT所致肝损伤小鼠的保护作用

Protective Effect of Lagotidis Herba on ANIT-induced Liver Injury in Mice

摘要:

目的：研究洪连对α-异硫氰酸萘酯（ANIT）所致黄疸型肝损伤小鼠的保护作用，并探讨其作用机制。方法：采用灌胃ANIT（100 mg·kg^-1）花生油溶液造成小鼠急性肝损伤模型，连续给药7 d，末次给药1 h后取血、肝脏，检测血清中谷氨酸-丙酮酸转氨酶（ALT），天门冬氨酸转氨酶（AST），总胆汁酸（TBA），总胆红素（TBIL）和肿瘤坏死因子-α（TNF-α）的含量；检测肝组织匀浆中超氧化物歧化酶（SOD），丙二醛（MDA），谷胱甘肽过氧化物酶（GSH-Px）的含量，PCR检测肝组织中TNF-α mRNA的表达量，苏木素-伊红（HE）染色观察肝组织病理变化。结果：与模型组比较，洪连（1.0，2.0，4.0 g·kg^-1）可显著降低肝损伤小鼠血清ALT，AST，TBIL和TNF-α的水平和肝匀浆中MDA含量，同时显著升高小鼠肝匀浆中GSH-Px的含量，洪连（2.0，4.0 g·kg^-1）可显著降低肝损伤小鼠血清TBA的水平和显著升高肝匀浆中SOD含量（P<0.01），洪连（1.0 g·kg^-1）组TBA，SOD含量与模型组比较无统计学差异。洪连提取物各剂量组均可显著降低肝组织中TNF-α mRNA相对表达量（P<0.01）。病理切片显示洪连（2.0，4.0 g·kg^-1）组能显著改善肝组织的病理变化。结论：洪连对ANIT所致小鼠黄疸型肝损伤有保护作用。

Abstract:

Objective: To study the protective effect of Lagotidis Herba on alpha-naphthyl isothiocyanate (ANIT) induced acute liver injury in mice, and investigate its mechanism of action. Method: The acute liver injury models were induced by intragastric administration of ANIT(100 mg·kg^-1). All groups were administered for 7 d, their serum and liver were collected 1 h after the last administration to detect the levels of Alanine transaminase (ALT), aspartate transaminase (AST), total bile acid (TBA), total bilirubin (TBIL) and tumor necrosis factor-α (TNF-α) in serum, the contents of superoxide dismutase (SOD), malonic dialdehyde (MDA) and glutathione peroxidase (GSH-Px) in liver homogenate, and the expression levels of TNF-α mRNA in liver tissues. The pathological changes of the liver tissues were observed by hematoxylin and eosin (HE) staining. Result: As compared with model group, various doses of Lagotidis Herba(1.0, 2.0 and 4.0 g·kg^-1) significantly decreased the levels of ALT, AST,TBIL and TNF-α in blood serum and the activity of MDA in liver homogenate, improved the activity of GSH-Px in liver homogenate, 2.0,4.0 g·kg^-1 Lagotidis Herba significantly decreased the level of TBA in blood serum, improved the activity of SOD in liver homogenate (P<0.01). As compared with model group, the contents of TBA and SOD of Lagotidis Herba(1.0 g·kg^-1) had no statistical difference in 4.0 g·kg^-1 Lagotidis Herba group. All doses of Lagotidis Herba significantly decreased the relative expression level of TNF-α mRNA in liver tissue (P<0.01). Furthermore, Lagotidis Herba (2.0, 4.0 g·kg^-1) could ameliorate the hepatic pathological changes. Conclusion: Lagotidis Herba has protective effects on acute hepatic injury induced by ANIT in mice.