黔产莪术油对人直肠癌细胞血管生成因子表达的影响

Effect of Zedoary Turmeric Oil from Guizhou on Expression of Angiogenesis Factors in Human Colorectal Cancer SW1463 Cells

摘要:

目的：通过黔产莪术油对人直肠癌SW1463细胞血管内皮生长因子（VEGF）和趋化因子（chemokine，CXC）蛋白表达的影响，探讨莪术挥发油对肿瘤血管生成的作用机制。方法：将经水蒸气蒸馏提取的黔产莪术挥发油，配制成80，120，160，200 mg·L^-1不同质量浓度，干预直肠癌SW1463细胞24 h后，倒置显微镜下观察不同浓度莪术油对直肠癌SW1463细胞的形态学影响；采用免疫细胞化学和蛋白免疫印迹法（Western blot）检测直肠癌SW1463细胞中VEGF，白细胞介素-8（IL-8）和CXC趋化因子受体2（CXCR2），CXC趋化因子受体3（CXCR3）蛋白的表达。结果：经莪术油处理直肠癌SW1463细胞24 h后，与空白组比较，莪术挥发油组细胞中VEGF，IL-8，CXCR2蛋白表达量明显下调（P<0.05，P<0.01），莪术油能抑制肿瘤细胞中VEGF，IL-8，CXCR2蛋白的表达；莪术挥发油组细胞中CXCR3蛋白表达量明显上调（P<0.05，P<0.01），莪术油能促进肿瘤细胞CXCR3蛋白的表达。结论：黔产莪术油可能通过下调促血管生成相关因子的表达，上调抑制性血管生成相关趋化因子的表达，从而抑制肿瘤血管生成，起到抑制肿瘤细胞的增殖作用。

Abstract:

Objective: To explore the mechanism of zedoary turmeric oil on tumor angiogenesis through the research of zedoary turmeric oil effect on vascular endothelial growth factor (VEGF) and chemokine(CXC) protein expression levels in rectal cancer SW1463 cells. Method: Steam distillation was used to extract volatile oil from Rhizoma curcuma, and prepared into different concentrations 80, 120, 160 and 200 mg·L^-1. After 24 h treatment on rectal cancer SW1463 cells, their effects on morphology of rectal cancer SW1463 cells were observed by inverted microscope. The protein expression levels of VEGF, CXC, interleukin(IL)-8 and its receptors CXCR2, CXCR3 in rectal cancer SW1463 cells were detected by immunocytochemistry and Western blot. Result: After treatment on rectal cancer SW1463 cells for 24 h by zedoary turmeric oil, the protein expression levels of VEGF, IL-8 and CXCR2 in cells were significantly down-regulated as compared with the normal blank group (P<0.05,P<0.01). The protein expression levels of VEGF, IL-8, CXCR2 could be inhibited in tumor cells by zedoary turmeric oil. However, the protein expression level of CXCR3 in cells was significantly up-regulated as compared with the normal blank group (P<0.05,P<0.01). The protein expression level of CXCR3 can be promoted in tumor cells by zedoary turmeric oil. Conclusion: Different concentrations of zedoary turmeric oil from Guizhou can inhibit rectal cancer SW1463 cells proliferation and alter cellular morphology. Zedoary turmeric oil from Guizhou can inhibit tumor angiogenesis through down-regulation of pro-angiogenesis-associated factors VEGF, IL-8, CXCR2 and up-regulation of angiogenesis-related chemokines CXCR3.