香鳞毛蕨有效部位乳膏对糠秕马拉色菌所致豚鼠感染模型的影响

Therapeutic Effect of Dryopteris fragrans Effective Parts Ointment in Malassezia furfur-infected Skin Model in Guinea Pigs

摘要:

目的：评价香鳞毛蕨有效部位乳膏（DF乳膏）对糠秕马拉色菌所致豚鼠感染模型的治疗作用。方法：构建糠秕马拉色菌豚鼠皮肤感染模型，按皮损评分值随机分成模型组，酮康唑组（1.33 mg·g^-1），DF乳膏高、中、低剂量组（含生药2，1，0.5 g·g^-1），通过测定肉眼评分、菌落计数（CFU），真菌学转阴率及组织病理检查4项指标，评价DF乳膏对糠秕马拉色菌所致豚鼠感染模型的治疗作用。结果：与模型组比较，DF乳膏高、中、低剂量组均可显著降低糠秕马拉色菌所致感染模型豚鼠皮损评分（P<0.05），明显减少病变皮肤菌落形成数（P<0.05），提高真菌培养的转阴率（P<0.05，P<0.01）；皮肤病理切片结果表明DF乳膏高、中剂量可明显减少感染组织皮肤角质层孢子数。结论：DF乳膏对糠秕马拉色菌所致的豚鼠感染有较好的治疗作用。

Abstract:

Objective: To assess the antifungal effect of Dryopteris fragrans effective parts ointment(DF ointment) in the Malassezia furfur-infected skin model in guinea pigs. Method: Guinea pigs infected with M. furfur for 7 days were divided into 5 groups on the 8^th day according to skin lesion scores:namely model group, ketoconazole group (1.33 mg·g^-1), high-dose, moderate-dose and low-dose DF ointment groups (containing 2, 1, 0.5 g·g^-1 of crude herb). The groups were treated with corresponding drugs for 10 days, qd. The efficacy of DF ointment was evaluated through observation of changes in skin lesion score, CFU number, negative rate and PAS staining. Result: High-dose, moderate-dose and low-dose DF ointment groups showed lower skin lesions(P<0.05) and CFU number (P<0.05) than model group, and higher negative rate (P<0.05, P<0.01). PAS staining revealed fewer spores in High-dose and moderate-dose DF ointment groups than model group. Conclusion: DF ointment has a better therapeutic effect on M. furfur-infected skin model in guinea pigs.