4种不同药材来源郁金饮片中挥发油成分的GC-MS分析

Analysis of Volatile Oil in Curcumae Radix Pieces from Four Different Sources by GC-MS

摘要:

目的：分析4种不同药材来源郁金饮片中挥发油成分，找出其共有及差异成分，为该饮片的合理使用提供依据。方法：采用水蒸气蒸馏法提取挥发油，利用GC-MS对4种不同药材来源郁金饮片挥发油成分进行测定，载气高纯度氦气，进样温度250 ℃，压力49.5 kPa，总流量36.0 mL·min^-1，柱流量1.0 mL·min^-1，线速度36.1 cm·s^-1；电离方式电子轰击离子源，电离电压70 eV，离子源温度200 ℃，接口温度280 ℃，溶剂延时3.5 min，m/z 40~400。结果：4种不同来源郁金饮片挥发油均检测出了50种成分，其中有3种共有成分，3种郁金共有成分达13种，2种郁金共有成分达15种；4种郁金共有、交互存在的挥发油成分达31种。结论：4种不同药材来源郁金饮片挥发油中存在共有及交互存在的成分。吉马酮可作为温郁金、桂郁金、绿丝郁金的含量测定指标成分；姜黄素、姜黄酮、芳姜黄酮等可作为黄丝郁金的含量测定指标成分。

Abstract:

Objective: To analyze volatile oil in Curcumae Radix pieces from four different sources and find out the common and different components. Method: The volatile oil in Curcumae Radix pieces from four different sources were extracted by steam distillation and determined by GC-MS,carrier gas was helium,temperature was rose by program,ionization mode was electron bombardment ion source. Result: Fifty components were determined in volatile oil in Curcumae Radix pieces from four different sources and three common components were found in four Curcumae Radix pieces;thirteen common components were found in three Curcumae Radix pieces;fifteen common components were found in two Curcumae Radix pieces;thirty-one components were found in two or more Curcuma Radix pieces,and the proportion was 62%. Conclusion: Curcumae Radix pieces from four different sources have common and interactive components in volatile oil.