均匀设计 偏最小二乘回归结合因子分析探索大黄 厚朴组分配伍的规律

Exploration of Compatibility Regularity of Components in Rhei Radix et Rhizoma and Magnoliae Officinalis Cortex by Uniform Design-partial Least Squares Regression Combined with Factor Analysis

摘要:

目的：采用均匀设计和偏最小二乘回归结合因子分析考察大黄-厚朴中大黄素、芦荟大黄素、厚朴酚及和厚朴酚4种成分的组方配伍规律。方法：健康雄性SD大鼠160只，随机分为8个组，依次为假手术组、模型组及四因素六水平均匀设计的6个配方组。胰胆管内逆行注射3.5% 牛磺胆酸钠法建立急性胰腺炎大鼠模型。术后6，24 h分别检测血清淀粉酶（AMS），胰脂肪酶（PL），白细胞介素-6（IL-6），IL-10，肿瘤坏死因子-α（TNF-α）的含量变化。偏最小二乘回归分析探索各因素对各药效指标的影响规律，因子分析抽提多项指标中的公共因子。结果：各组分对急性胰腺炎病症的贡献度排序为厚朴酚>和厚朴酚>大黄素>芦荟大黄素。因子分析抽提出隐藏在多项指标后的公共因子为胰腺损伤因子（AMS，IL-6和TNF-α）和炎症因子（PL和IL-10）。结论：采用均匀试验设计、偏最小二乘回归结合因子分析可有效地分析组分配伍中多因素与多效应成分之间的关联规律，为临床病症的治疗提供方-症更为对应的组分配方。

Abstract:

Objective: To investigate the compatibility regularity of components (emodin,aloe-emodin,magnolol and honokiol) in Rhei Radix et Rhizoma and Magnoliae Officinalis Cortex by uniform design and partial least squares regression combined with factor analysis. Method: SD rats were randomly divided into 8 groups,including sham-operated group,model group and six compatibility groups.The rat model of acute pancreatitis was established by the retrograde injection of 3.5% sodium taurocholate.Abdominal aortic blood was drawn at 6 hour and 24 hour for determination of serum amylase(AMS),pancreatic lipase(PL),interleukin-6(IL-6),IL-10 and tumor necrosis factor-α(TNF-α).The partial least squares regression was adopted to explore the influence law of factors on efficacy index,and factor analysis was used to extract the public factors in indexes. Result: The influence degree of each component on acute pancreatitis was in the order of magnolol>honokiol>emodin>aloe-emodin.Factor analysis extracted two public factors,such as pancreatic injury factors(AMS,IL-6 and TNF-α) and inflammatory factors(PL and IL-10). Conclusion: This method,uniform design-partial least squares regression combined with factor analysis,can effectively clarify the correlation and regularity between many factors and many components,which can provide more suitable effect formulas for clinical symptoms.