不同炮制工艺山绿茶药材特征图谱的比较研究

Comparative Study on the Characteristic Chromatogram of Different Processing Methods of Ilex hainanensis Merr.

摘要:

[目的]采用HPLC法研究不同炮制工艺山绿茶药材特征图谱的变化,为科学评价和控制其质量提供有效的方法。[方法]以70%乙醇为提取溶剂,采用加热回流的方法提取制备供试样品。以Phenomenon C18色谱柱为固定相,乙腈-0.1%磷酸水溶液为流动相进行梯度洗脱,检测波长330nm,柱温25℃,进样量10μl,流速1.0ml/min,进行HPLC特征图谱检测。以芦丁为对照品,检测不同炮制工艺药材芦丁含量变化。[结果]不同炮制工艺药材中共确定9个主要共有特征峰。研究发现特征图谱中主要色谱峰发生了显著改变,与炮制前相比峰面积显著降低。表明山绿茶药材经炮制处理后,主要化学成分含量明显减少。[结论]建立了一种重现性、精密度良好的HPLC特征图谱检测方法,可用于山绿茶药材及其炮制品的质量控制。

Abstract:

Objective To establish a sensitive and specific HPLC method for quality control of Ilex hainanensis Merr.．Methods HPLC method was applied for quality assessment of Ilex hainanensis Merr.． HPLC analysis was performed on a HanbonC18 column (250mm×4．6mm，5 m)．The mobile phase consisted of acetonitrile (solvent B) and watercontaining0.1% (v/v) phosphoric acid (solvent A) at a constant flow rate of 1.0ml/min．An increasing linear gradient (v/v) of solvent B was used. The injection volume was 10μl. The column temperature was set at 25℃. The chromatogram s were monitored at 330nm. Results In different varieties of processing methods of Ilex hainanensis Merr. showing 9 characteristic peaks. It was established from its prepared samples of Ilex hainanensis Merr.．Conclusion The characteristic chromatogram of Ilex hainanensis Merr. with high specificity can be used to control its quality as well as the consistency of processing products.