Abstract:Objectivt:To establish a determining method for Tetramethylpyrazine(TMP)in Fufang Chuanxiong Capules by high-perfomance liquid chromatography.Methods:Tetramethylpyrazine was extracted from Fufang Chuanxiong Capules by 80% methanol.Nova-Pak C_{18 colum(3.9mm×300mm,4μm) was used as stationary phase,methanol-H 2O(50:50v/v)was used as mobile phase,the flow rate was 0.7mL·min-1 and the wavelength of detection was at 282nm.Results:The calibration curve was showed good linearity within the concentration range of 5～50 μg·mL^-1 r=0.9996,the average recovery was 99.0%,RSD=1.2%.Conclusion:The established method is accurate,precise and simple.}

Abstract:Objectives: To determine the content of chlorogenic acid in Yinqiaojiedu Granuces by HPLC. Method: The analysis was performed on an hypersil C18 column, with a mobile phase of methanol-water-acetic acid-glacial-triethylamine (15:85:1:0.2), the detection wavelength was 254nm. Result: Recovery of chlorogenic acid was 98.12% RSD=0.97%(n=5). Conclusion:This method is simple and reproducible, and it can be used for the quantitative analysis of chlorogenic acid in Yinqiaojiedu Granuces.

Abstract:Objective:To explore the influence of two kinds of active components from Panax notoginseng on NO and iNOS on inflammatory Metrorrhagia model of Macaca multatta. Methods:Eleven normal menorrhea adult Rhesus monkeys were choosed as research object,and were divided into model group (four),TCM group(four) and Control group(three) at random.Colorimetric method were performed to estimate the activity of NO and iNOS in of model group,TCM group and normal control group,the immunohistochemical S-p were performed to estimate the express level of iNOS in the endometrium of the three groups.Result:Compared to the model group,the activity of No and iNOS and the express level of iNOS in the endometrium of TCM gorup was obviously weaker,which are similar to that of the normal group.Conclusion:NO may be involred in the pathogenesis of inflammatory Metrorrhagia.Two kinds of active components from Panax notoginseng reduce inflammatory Metrorrhagia by regulating the activities of NO and iNOS and the express level of iNOS in the endometrium.

Abstract:We studied the effects of salvianolic acid B(Sa1.B)、Rb、Re and Rg fractions of Danshen or Sanqi on platelet aggregation and adhesion,TXA₂ and PGI₂ in normal rats. The results demonstrated that Re fraction (68mg/kg)and Rg fraction (108mg/kg),given orally,could markedly counteract ADP induced platelet aggregation.Rg fraction (108mg/kg) and Sal.B fraction(48mg/kg) could markedly inhibit platelet adhesion.Rb fraction(112mg/kg) have no effects on platelet aggregation and on adhesion.Rg fraction(108mg/kg) and Sal.B fraction (48mg/kg) could markedly decrease the level of TXA₂,but had little effect on 6-Keto-PGF_1α. Moreover, Rg fraction 108mg/kg could decrease the ration of TXB₂/6-Keto-PGF_1α,too. The results suggested that the inhibition effects on platelet aggregation and adhesion of Rg fraction(108mg/kg) and Sal.B fraction(48mg/kg) of Danshen or Sanqi might be closely related to its inhibition effect on the release of TXA₂ in normal rats.

Abstract:Objective To study the pharmacological effects of ChuanBeiPipa Confection.Methods The anti-tussive,anti-asthmatic and expectorant effects were observed by concentrated ammonia method in mice,spray method in cavies and capillary method in mice. Results ChuanBeiPipa Confection had inhibitory effect on cough caused by concentrated ammonia in mice,prolonged the latency of cough,decreased the frequency of cough,and increased the secretion of expectoration.The confection also had the significant inhibitory effects on asthma in cavies. Couclusion ChuanBeiPipa Confection has anti-tussive,anti-asthmatic and expectorant effects.

Abstract:Objective: To observe the Pharmacological effect of the sediments produced by matching of Herbs in Xiexintang.Method:Gastric ulcer model of rat induced by polorous ligation with aspirin administration model of rat induced by carragreenin were used.Bactriostatic effect was evaluated by using gold staphylococcus and Escherichia.Effects of Xiexin decoction,the supemate and the sediments of the decoction were compared by using the above mentioned models and bacterial cultuer.Result:The effects of the sediments of Xiexin decoction and that of the Xiexin decoction are similar.Conclusion:Biological activity of the sediments that produced by matching of herbs of Xiexintang decocting and that of the Xiexin decoction are the same.

Abstract:Objective To investigate the effects of assemble flavones of Fortune's drynaria rhizoma on animal bone mineral density,phasma calcium and phosphorus, and on analgesia action.Methods Wistar rat and Kunming mouse were used as experimental animals. Osteoporosis was induced by orally given retinoic acid(70 mg·kg^-1·d^-1) to the rats for 14 dasy. For evaluation of analgesia action,body wresting was induced by vinegar id injection in the mice, and hot plate method(55℃) of mouse was employed. Results Bone mineral density and plasma calcium were significantly increased for the dose groups of Fortune's drynaria zhizoma compared with the control(P<0.05).Analgesia action evaluated by vinegar induced and heat induced pain was also significant compared with the control(P<0.05).Conclusion The assemble flavones of Fortune's drynaria rhizoma have the functions of elevating blood calcium, increasing bone mineral density and analgesia action.

Abstract:Objective:To study the pharmacological functions of Mangan Yangyin Capsule.Methods:Rat was selected to conduct the experiment on chronic hepatic injury caused by carbon tetrachloride.Mouse was used to conduct the experiments for stagnation of the liver-qi and deficiency of the spleen.Mouse was also used for the acute hepatic injury caused by chemicals and the immunity experiments.Results:Mangan Yangyin Capsules obviously improved the hepatic dysfunction,decreased the contents of triglyceride and cholesterol,enhanced the immunoreaction in mice, and raised peritoneal macrophage phagecytosis. Conclusion:Mangan Yangyin Capsule had the protective effects for liver function and nourishing function to liver and kidney.

Abstract:Objective:observe the effect of Guben Zhike Gao on expelling phlegm,relieving coungh and asthma and resisting inflammation.Methods:The coungh-relieving effect of Guben Zhike Gao was observed by mouse cough model induced by SO₂ and NH₄OH with Capillary method. Observation on the phlegmexpelling effect was completed by using phenol red. Observation on the asthma-relieving effect was completed by inducing asthma with Ach-histamine method in quinea pig. The anti-inflammation effect of Guben Zhike Gao was investigated by implantation of carrageenin and cotton ball in rat. Results:The Guben Zhike Gao can prolong apparently the coughing incubation period and reduce coughing frequency;increase excretion of phenol red and phlegm from the respiratory tract; prolong the incubation period of asthma due to Ach-histamine solution and resist inflammation on acute and chronic inflammator models. Conclutions:The Guben Zhike Gao has the functions of expelling phlegm,relieving cough and asthma,resisting inflammation.

Abstract:Objective:To explore the inhibitory effect of tumor growth and antiangiogenesis of Gubenxiaoliu capsule(GC) on Lewis lung carcinoma of mouse.Methods:In vivo animal experiment was used to investigate the growth of mouse tumor.Immunohistochemically(S-P) and quantitative pathologic image analysis were used to investigate the microvesel dencity(MVD) and expression of vascular endothelial growth factor(VEGF) in tumor tissue.Results:The inhibitory rates on mouse tumor of GC group,Chemotherapy group and GC+Chemotherapy group are 40.58%, 52.69%, 61.09% respectively,The inhibitory rates are significantly higher than the control,MVD and expression of VEGF of GC group and GC+Chemotherapy group and MVD of Chemotherapy group decreased significantly.Conelusion:GC could inhibit the growth and angiogenesis of Lewis lung carcinoma of mouse.

Abstract:Disease name diagnosis is the core matter in clinical diagnosis of Traditional Chinese Medicine. The name of Hepatolenticular degeneration has a lot of problems from a scientific, standar dization and normalization point of view. Based on classical literatures, the authors discuss the shortcoming of the disease name and suggest to replace "hepatolenticular degeneration" by "Wilson's disease".